Image credits Developing eye of zebrafish labelled with phalloidin TRITC. Courtesy to Madhangi M., Upendra Nongthomba Lab

Matching your wavelength with the nano-scale

The Bio-Imaging Facility, Division of Biological Sciences, offers advanced microscopy solutions for a wide range of biological imaging applications. Equipped with state-of-the-art confocal, super-resolution, TIRF, FLIM, FRAP, and laser capture microdissection system, the facility enables high-resolution imaging and quantitative analysis of biological specimens, from cells and organelles to molecular interactions, in both live and fixed samples.

The facility provides confocal laser scanning microscopy (CLSM) services to various Departments and Centers of the Institute, primarily the Division of Biological Sciences. Besides this, various investigators from Engineering and Basic Sciences such as the Materials Research Centre (MRC), Materials Engineering (ME), Mechanical Engineering, Inorganic Physical Chemistry (IPC), Physics, Instrumentation and applied physics, Organic Chemistry, and Nanoscience (CeNSE) use our facility. Our facility is also open to external users both from academia and corporate institutions (from November, 2016). We have dedicated and trained personnel operating the microscopes who have experience imaging bacteria, mammalian cells, yeast cells, Drosophila and Zebra fish, sections of brain and other tissues, carbon nanodots and nanoparticles, in both fixed and live specimens. 

Booking System

BOOKING AND FACILITY USAGE

The facility operates under established user guidelines to ensure efficient and safe instrument usage. For registration and access, users may contact the facility at confocaliscs@gmail.com or bioimaging.bio@iisc.ac.in. Instrument reservations are managed through the Facility Booking Portal https://bookings-bioif.iisc.ac.in

Confocal live cell imaging of calcium fluxes in cardiomyocyte like stem cells, imaged on the LSM 880 confocal microscope, Courtesy, Aditi Jain, Ravi Sunderesan Lab

Contact:

The Bioimaging Facility
Ground Floor, C-Wing,
GC-01 and 02
Division of Biological Sciences
Indian Institute of Science
Bangalore-560012
080 22933506
bioimaging.bio@iisc.ac.in / confocaliisc@gmail.com

Image credits: Numbering from left to right

Image 1: Drosophila larval brain stained with FITC and TRITC, Courtesy to Ruchi Jain., Upendra Nongthomba Lab

Image 2: Developing embryo of zebrafish labelled with phalloidin TRITC and FITC, Courtesy to Madhangi M., Upendra Nongthomba Lab

Image 3: Mitochondria in Bovine  Aortic Endothelial cells expressing mtDsRed and DAPI staining nucleus, Courtesy to Lekha E.M., Sandeep M Eswarappa Lab

Image 4: Whole Arabidopsis leaf stained with analine blue showing autofluorescence, Courtesy to Kavita Rao., Utpal Nath Lab

Image 5: DIC image of tardigrade species isolated from IISc campus, Courtesy to Hari Kumar., Sandeep M Eswarappa Lab

Image 6: Convallaria majalis rhizome section showing autofluorescence. Courtesy to Carl Zeiss, India

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