Leica TCS SP5 Confocal microscope, upright

Detailed specifications: This microscope is equipped with a high speed scanner (a resonant scanner) which works at more than 25 fps, ideally meant for live cell imaging. It has three conventional detectors for fluorescence imaging and one for transmitted light, therefore the system can be used well for routine multi-dye imaging.

Lasers:

  • Diode laser 405 nm
  • Multi Argon laser (458, 476, 488, 496, 514 nm)
  • DPSS 561 nm
  • HeNe- laser 633nm

Objectives:

  • 10x/0.30 water, HCX APO L U-V-I
  • 40x/0.80 water , HCX APO L U-V-I
  • 63x/0.9 water,  HCX APO L U-V-I
  • 63x/1.4 oil: HCX PL APO lambda blue

Detectors:

  • Three channels 3 PMTs
  • Transmitted light detector PMT Trans

Computer:

  • MultiSync LCD workstation with Windows 2007
  • Leica LAS AF SP5 software is used for imaging and analysis.
Image credits: Numbering from left to right

Image 1: Embryonic stem cell derived fibroblasts showing constitutive eGFP expression (green) and Sca-1 (red) and DNA stained with DAPI, Courtesy to Divya S., PB Seshagiri Lab

Image 2: Astrocytes stained with glial fibrillary acidic protein (GFAP) and DNA with propidium iodide (PI), Courtesy to Isha Verma, PB Seshagiri Lab

Image 3: Mitochondria in Bovine Aortic Endothelial cells expressing mtDsRed and DAPI staining nucleus, Courtesy to Lekha E.M., Sandeep M Eswarappa Lab

Image 4: THP-1 cells infected with Mrx1 ro GFP2 MTB cells (Green) co-localised in lysosome (yellow, early and red, late), Courtesy to Santosh Poddar, Amit Singh Lab

Image 5: Courtesy to Gowri, Annapoorni Rangarajan Lab

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