Zeiss LSM 510 Meta

Detailed specifications: Laser scanning confocal microscope (LSCM) with META detector module which can scan across a range of wavelengths to allow digital separation of overlapping spectra (such as GFP and YFP). The achievable scan speed is from a range of 5 fps to 70 fps for 512x 512 pixel resolution.

Lasers:

• Argon laser (458, 477, 488, 514 nm, 30 mW)
• HeNe- laser 543 nm, 1mW (Integrated)
• HeNe- laser 633nm, 5mW (Integrated)

Fluorescence source:

• Lamphousing, Halogen 10v/100W, Bulb 12V square filament
• Lamp Unit HBO 103 w/o Ignition Device, Burner HBO 103

Conventional fluorescence filters:

• Confocal detection channel 2 for LSM 510
• Confocal detection channel 3 for LSM 510
• Green and Red

Objectives:

• 10x/0.45, Objective Plan-Apochromat WD= 2.1 mm
• 20x/0.75 Objective Plan-Apochromat WD= 0.61 mm
• 40x/0.95 Objective Plan-Apochromat  Corr WF= 0.16 mm
• 40x/1.20 water: Objective C-Apochromat  Corr WD= 0.22 mm
• 63x/1.3 oil: Objective Plan-Apochromat  oil DIC
• 100x/1.3-oil: Objective Plan-Apochromat  Corr

Detectors:

• Three channels
• Meta detection module
• All cooled PMTs
• Integration of DC scanning stage “Axiovert 200”
• Axiocam for video mode of imaging

Computer:

• Host computer LSM 510/LSM5 PASCAL/FCS
• LCD TFT Display monitor P19-1 (19’’)
• DVD + RW Recorder ATAPI
• Additional hard disk 120 GB Ultra DMA100
• Memory 1024 MB DDR-RAM
• LSM –META software is used for imaging and analysis.